Semi Prep HPLC Column, RP C4 Wide Pore, 5um, 300A. 10mm ID x 150mm Length. Cogent Brand. 1 EA. Special Order Item.
Additional Information:
Cogent RP C4™ Wide Pore is a reversed-phase HPLC stationary phase designed for the purification and isolation of proteins, peptides, enzymes, and other biological compounds. Manufactured using a four-carbon butyl ligand bonded to ultra-high purity silica and fully double end capped, this 300 Å wide pore stationary phase provides enhanced accessibility for large biomolecules while maintaining the lower hydrophobic retention characteristics of C4 chemistry. The wide pore design is particularly beneficial when purifying larger molecules that require greater pore accessibility and efficient mass transfer.
68504-SP150-3M is a semi-preparative HPLC column packed with 5 µm RP C4™ Wide Pore particles featuring a 300 Å pore size in a 10 mm ID x 150 mm format. The 10 mm internal diameter provides significantly greater sample loading capacity than analytical columns, while the 150 mm length provides increased chromatographic resolution and peak capacity compared to shorter semi-preparative formats. The 300 Å pore structure improves accessibility for proteins, peptides, enzymes, and other biomolecules. Typical operating flow rates are approximately 4–8 mL/min depending on mobile phase composition, analyte properties, and system capabilities.
This column is ideally suited for semi-preparative purification of proteins, peptides, enzymes, and other biological compounds in pharmaceutical, biopharmaceutical, and research laboratories. Compared to more hydrophobic C8 and C18 phases, the C4 bonded phase generally provides lower retention, improving recovery and purification efficiency for larger biomolecules. A semi-preparative HPLC system capable of the required flow rates is recommended for optimal performance.
Key Benefits
RP C4™ Wide Pore stationary phase
USP L26 equivalent chemistry
Butyl (C4) bonded phase
Fully double end capped
Ultra-high purity silica support
300 Å wide pore design
Increased sample loading capacity
Optimized for proteins and peptides
Lower hydrophobic retention than C8 phases
Enhanced semi-preparative resolution
Typical Applications
Semi-preparative HPLC
Protein purification
Peptide purification
Enzyme purification
Biomolecule isolation
Biopharmaceutical analysis
Fraction collection
Method development
Process development
Research and development
Specifications
Product Type: Semi-Preparative HPLC Column
Stationary Phase: RP C4™ Wide Pore
USP Classification: L26
Chromatography Mode: Reversed Phase
Bonded Phase: Butyl (C4)
Endcapping: Fully Double End Capped
Particle Size: 5 µm
Pore Size: 300 Å
Column Dimensions: 10 mm ID x 150 mm
Length: 150 mm
Typical Flow Rate: 4–8 mL/min
Brand: Cogent
Special Order Item Information
Important Notes
Designed for purification of proteins, peptides, enzymes, and other biological compounds
300 Å wide pores improve accessibility for larger biomolecules
Provides less retention than C8 and C18 stationary phases
Ideal for semi-preparative purification and fraction collection
The 10 mm ID format offers increased loading capacity compared to analytical columns
Typical operating flow rates are approximately 4–8 mL/min
5 µm particles provide lower operating backpressure and robust performance
Fully double end capped to minimize secondary interactions
The 150 mm length provides increased resolution and peak capacity compared to shorter semi-preparative columns
Requires an HPLC system capable of semi-preparative flow rates